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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
08/01/1997 |
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Data da última atualização: |
22/06/2023 |
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Autoria: |
BAR-SAGIE, D.; MAYEVSKY, A.; BARTOOV, B. |
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Título: |
A fluorometric-reflectometric technique for simultaneous measurement of pH and motility in ram semen. |
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Ano de publicação: |
1981 |
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Fonte/Imprenta: |
Archivos of Andrology, v. 7, n. 1, p. 27-33, Aug. 1981. |
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DOI: |
10.3109/01485018109009372. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: An optical technique for simultaneous assessment of pH and motility in ram semen was described. Motility was evaluated by the measurement of changes in intensity of reflected light from semen samples illuminated with 366 nm light. pH was measured by using a pH-sensitive fluorescent indicator (umbelliferone). From the changes in fluorescence intensity (450 nm) as a function of the pH of the semen, a calibration curve was constructed. The relationship between pH changes and motility in various metabolic conditions were described. Different kinetics for pH changes occurred when sperm motility was driven by both mitochondrial respiration and fructolysis or by either one of them. An analysis of the fluorescence signal from extracellular or cellular fractions of semen suggested that the pH measurement reflected extracellular pH. |
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Palavras-Chave: |
Analysis; Bioquimica seminal; Hydrogen-Ion Concentration; Motilidade. |
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Thesagro: |
Ovino; Reprodução; Sêmen. |
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Thesaurus Nal: |
Males; Reproduction; Sheep; Sperm motility; Umbelliferones. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 01691naa a2200301 a 4500
001 1523640
005 2023-06-22
008 1981 bl uuuu u00u1 u #d
024 7 $a10.3109/01485018109009372.$2DOI
100 1 $aBAR-SAGIE, D.
245 $aA fluorometric-reflectometric technique for simultaneous measurement of pH and motility in ram semen.$h[electronic resource]
260 $c1981
520 $aAbstract: An optical technique for simultaneous assessment of pH and motility in ram semen was described. Motility was evaluated by the measurement of changes in intensity of reflected light from semen samples illuminated with 366 nm light. pH was measured by using a pH-sensitive fluorescent indicator (umbelliferone). From the changes in fluorescence intensity (450 nm) as a function of the pH of the semen, a calibration curve was constructed. The relationship between pH changes and motility in various metabolic conditions were described. Different kinetics for pH changes occurred when sperm motility was driven by both mitochondrial respiration and fructolysis or by either one of them. An analysis of the fluorescence signal from extracellular or cellular fractions of semen suggested that the pH measurement reflected extracellular pH.
650 $aMales
650 $aReproduction
650 $aSheep
650 $aSperm motility
650 $aUmbelliferones
650 $aOvino
650 $aReprodução
650 $aSêmen
653 $aAnalysis
653 $aBioquimica seminal
653 $aHydrogen-Ion Concentration
653 $aMotilidade
700 1 $aMAYEVSKY, A.
700 1 $aBARTOOV, B.
773 $tArchivos of Andrology$gv. 7, n. 1, p. 27-33, Aug. 1981.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
